aav based vectors packaged into aav9 viruses Search Results


aav9 sico mouse gdf15 shrna  (Vector Biolabs)
95
Vector Biolabs aav9 sico mouse gdf15 shrna
A Plasma IGF1 concentrations (ng/ml) in 7‐day‐old weight‐ and gender‐matched littermate WT mice injected with control or different proteins were measured by ELISA ( n = 3–5 mice per group, daily i.p. injection from 5 days of age). B–G Liver phosphorylated and total STAT5 and JAK2 as well as β‐actin (loading control) determined by Western blot (B); liver expression of Igf1 , Igfbp3, and Igfals quantified by qPCR (C); plasma IGF1 (D), IGFBP3 (E), and GH concentrations (F) measured by ELISA; and daily body weight (G) in weight‐ and gender‐matched littermate WT mice injected with control or <t>GDF15</t> ( n = 5 per group, daily i.p. injection from 3 days of age). H Overnight‐fasted (in DMEM) WT mouse primary hepatocytes were first treated with different concentrations of GDF15 for 30 min and then with 20 ng/ml GH for 15 min. Cellular levels of phosphorylated STAT5, total STAT5, and β‐actin (loading control) were determined by Western blot. Data information: * P < 0.05, ** P < 0.01, and *** P < 0.001 between control and GDF15 by t ‐test. All values are mean + s.e.m. Source data are available online for this figure.
Aav9 Sico Mouse Gdf15 Shrna, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A Plasma IGF1 concentrations (ng/ml) in 7‐day‐old weight‐ and gender‐matched littermate WT mice injected with control or different proteins were measured by ELISA ( n = 3–5 mice per group, daily i.p. injection from 5 days of age). B–G Liver phosphorylated and total STAT5 and JAK2 as well as β‐actin (loading control) determined by Western blot (B); liver expression of Igf1 , Igfbp3, and Igfals quantified by qPCR (C); plasma IGF1 (D), IGFBP3 (E), and GH concentrations (F) measured by ELISA; and daily body weight (G) in weight‐ and gender‐matched littermate WT mice injected with control or GDF15 ( n = 5 per group, daily i.p. injection from 3 days of age). H Overnight‐fasted (in DMEM) WT mouse primary hepatocytes were first treated with different concentrations of GDF15 for 30 min and then with 20 ng/ml GH for 15 min. Cellular levels of phosphorylated STAT5, total STAT5, and β‐actin (loading control) were determined by Western blot. Data information: * P < 0.05, ** P < 0.01, and *** P < 0.001 between control and GDF15 by t ‐test. All values are mean + s.e.m. Source data are available online for this figure.

Journal: EMBO Molecular Medicine

Article Title: GDF 15 is a heart‐derived hormone that regulates body growth

doi: 10.15252/emmm.201707604

Figure Lengend Snippet: A Plasma IGF1 concentrations (ng/ml) in 7‐day‐old weight‐ and gender‐matched littermate WT mice injected with control or different proteins were measured by ELISA ( n = 3–5 mice per group, daily i.p. injection from 5 days of age). B–G Liver phosphorylated and total STAT5 and JAK2 as well as β‐actin (loading control) determined by Western blot (B); liver expression of Igf1 , Igfbp3, and Igfals quantified by qPCR (C); plasma IGF1 (D), IGFBP3 (E), and GH concentrations (F) measured by ELISA; and daily body weight (G) in weight‐ and gender‐matched littermate WT mice injected with control or GDF15 ( n = 5 per group, daily i.p. injection from 3 days of age). H Overnight‐fasted (in DMEM) WT mouse primary hepatocytes were first treated with different concentrations of GDF15 for 30 min and then with 20 ng/ml GH for 15 min. Cellular levels of phosphorylated STAT5, total STAT5, and β‐actin (loading control) were determined by Western blot. Data information: * P < 0.05, ** P < 0.01, and *** P < 0.001 between control and GDF15 by t ‐test. All values are mean + s.e.m. Source data are available online for this figure.

Article Snippet: AAV9‐Sico‐mouse Gdf15 shRNA (based on shAAV‐260008) or scramble control shRNA was custom‐built and manufactured by Vector Biolabs.

Techniques: Clinical Proteomics, Injection, Control, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

Expression of Gdf15 in 3‐ ( n = 5–7 mice per group), 7‐ ( n = 6 mice per group), 10‐ ( n = 8–10 mice per group), and 13‐day‐old ( n = 12–13 mice per group) littermate mouse hearts quantified by qPCR. GDF15 protein level in 3‐, 7‐, 10‐, and 13‐day‐old littermate mouse hearts determined by Western blot. β‐Actin serves as a loading control. Plasma GDF15 concentrations in 3‐ ( n = 5–7 mice per group), 7‐ ( n = 6 mice per group), and 10‐day‐old littermate mice ( n = 8–10 mice per group) measured by ELISA. Representative pictures of 3‐, 7‐, 10‐, and 13‐day‐old littermate αHetγWT and αKOγKO mouse heart sections stained with GDF15 antibody (brown) and counterstained with hematoxylin (purple). Scale bar: 100 μm. Representative pictures of 16‐day‐old αKOγKO mouse hearts stained with GDF15 (red) and cardiac troponin I (green) antibodies. Arrows point to the nucleus of cardiomyocytes, and asterisks mark the nucleus of non‐cardiomyocytes. Scale bar: 20 μm. Data information: * P < 0.05, ** P < 0.01, and *** P < 0.001 between αKOγKO and all other littermate control genotypes by t ‐test. All values are mean + s.e.m.

Journal: EMBO Molecular Medicine

Article Title: GDF 15 is a heart‐derived hormone that regulates body growth

doi: 10.15252/emmm.201707604

Figure Lengend Snippet: Expression of Gdf15 in 3‐ ( n = 5–7 mice per group), 7‐ ( n = 6 mice per group), 10‐ ( n = 8–10 mice per group), and 13‐day‐old ( n = 12–13 mice per group) littermate mouse hearts quantified by qPCR. GDF15 protein level in 3‐, 7‐, 10‐, and 13‐day‐old littermate mouse hearts determined by Western blot. β‐Actin serves as a loading control. Plasma GDF15 concentrations in 3‐ ( n = 5–7 mice per group), 7‐ ( n = 6 mice per group), and 10‐day‐old littermate mice ( n = 8–10 mice per group) measured by ELISA. Representative pictures of 3‐, 7‐, 10‐, and 13‐day‐old littermate αHetγWT and αKOγKO mouse heart sections stained with GDF15 antibody (brown) and counterstained with hematoxylin (purple). Scale bar: 100 μm. Representative pictures of 16‐day‐old αKOγKO mouse hearts stained with GDF15 (red) and cardiac troponin I (green) antibodies. Arrows point to the nucleus of cardiomyocytes, and asterisks mark the nucleus of non‐cardiomyocytes. Scale bar: 20 μm. Data information: * P < 0.05, ** P < 0.01, and *** P < 0.001 between αKOγKO and all other littermate control genotypes by t ‐test. All values are mean + s.e.m.

Article Snippet: AAV9‐Sico‐mouse Gdf15 shRNA (based on shAAV‐260008) or scramble control shRNA was custom‐built and manufactured by Vector Biolabs.

Techniques: Expressing, Western Blot, Control, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Staining

A Design of AAV9‐mGDF15 shRNA construct to specifically knockdown GDF15 in Cre + cardiomyocytes. B–G Cardiac Gdf15 expression quantified by qPCR (B), plasma GDF15 concentrations measured by ELISA (C), cardiac Bnp expression quantified by qPCR (D), liver phosphorylated STAT5 level measured by ELISA (E), and plasma IGF1 (F) and GH concentrations (G) measured by ELISA in 9‐ to 10‐day‐old littermate control and αKOγKO mice ( n = 8–12 mice per group) that received pericardial injection of AAV9‐control or Gdf15 shRNA at 2 days of age. * P < 0.05, ** P < 0.01, and *** P < 0.001 by t ‐test. Values are mean + s.e.m.

Journal: EMBO Molecular Medicine

Article Title: GDF 15 is a heart‐derived hormone that regulates body growth

doi: 10.15252/emmm.201707604

Figure Lengend Snippet: A Design of AAV9‐mGDF15 shRNA construct to specifically knockdown GDF15 in Cre + cardiomyocytes. B–G Cardiac Gdf15 expression quantified by qPCR (B), plasma GDF15 concentrations measured by ELISA (C), cardiac Bnp expression quantified by qPCR (D), liver phosphorylated STAT5 level measured by ELISA (E), and plasma IGF1 (F) and GH concentrations (G) measured by ELISA in 9‐ to 10‐day‐old littermate control and αKOγKO mice ( n = 8–12 mice per group) that received pericardial injection of AAV9‐control or Gdf15 shRNA at 2 days of age. * P < 0.05, ** P < 0.01, and *** P < 0.001 by t ‐test. Values are mean + s.e.m.

Article Snippet: AAV9‐Sico‐mouse Gdf15 shRNA (based on shAAV‐260008) or scramble control shRNA was custom‐built and manufactured by Vector Biolabs.

Techniques: shRNA, Construct, Knockdown, Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, Injection

Plasma GDF15 concentrations in 2‐ to 3‐year‐old children diagnosed with heart disease with either normal body weight ( n = 35) or FTT ( n = 45) and in age‐ and gender‐matched healthy controls ( n = 45) were measured by ELISA. * P < 0.05 and *** P < 0.001 by t ‐test. Values are mean ± s.e.m. Cartoon illustrating how GDF15 and ANP/BNP relieve cardiac burden and coordinate cardiac function with the rest of the body.

Journal: EMBO Molecular Medicine

Article Title: GDF 15 is a heart‐derived hormone that regulates body growth

doi: 10.15252/emmm.201707604

Figure Lengend Snippet: Plasma GDF15 concentrations in 2‐ to 3‐year‐old children diagnosed with heart disease with either normal body weight ( n = 35) or FTT ( n = 45) and in age‐ and gender‐matched healthy controls ( n = 45) were measured by ELISA. * P < 0.05 and *** P < 0.001 by t ‐test. Values are mean ± s.e.m. Cartoon illustrating how GDF15 and ANP/BNP relieve cardiac burden and coordinate cardiac function with the rest of the body.

Article Snippet: AAV9‐Sico‐mouse Gdf15 shRNA (based on shAAV‐260008) or scramble control shRNA was custom‐built and manufactured by Vector Biolabs.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay